Hi ,I was wondering if anyone has tried this method with Diatom...

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ALISON HENDRY
Jul 24, 2018
protocol Protocol: Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
Hi ,

I was wondering if anyone has tried this method with Diatom cultures and also if anyone has tried using Acetone instead of Methanol as an extraction solvent?

Hope you can help,

Alison
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Maria A. Sinetova Author Answered Jul 24, 2018

Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences, Russia

Dear Alison,
I’ve never tried this protocol with diatoms, but based on experience with other algae which is in coincidence with data from thorough study of Ritchie (2006) methanol is a more efficient extractant of chlorophyll than 90% acetone, ethanol should work similarly as methanol. So for spectrophotometric quantitative estimation methanol/ethanol is a better choice. But chlorophylls are less stable in methanol and ethanol and tend to form allomers so for HPLC study it is preferable to use other solvents (acetone, chloroform etc.) and mechanical disruption will be needed in most cases.

Best regards,
Maria
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CLAUDE YEPREMIAN Answered Jul 24, 2018

MUSEUM NATIONAL D'HISTOIRE NATURELLE, UMR 7245

Dear Allison,

May I send you a protocol using ethanol instead methanol
Contact me at : claude.yepremian@mnhn.fr, I shall send you the pdf.
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