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Hello, I use this protocol to extract other plant leaf EVs,...
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chuan shen Jul 21, 2018
Hello, I use this protocol to extract other plant leaf EVs, but low fluorescence value was collected. I find there are few differences between this protocol and the paper, so I want to ask for your help.
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Brian D. Rutter Author Answered Jul 20, 2018
Indiana University
Hi Chuan Shen,
I hope you got my email addressing you question.
I’d like to help you, but I need more information. There are lots of reasons why you may be getting low fluorescence. It could be that you aren’t collecting enough vesicles, and you need to use more apoplastic wash in you experiments. It could be that you are losing your pellet during the centrifugation steps by using a different kind of tube or pipetting technique. Maybe your fluorometer has a different level of sensitivity or your DIOC has gone bad.
If you share your protocol with me. Maybe I can see what could be the problem.
Best of luck,
Brian
I hope you got my email addressing you question.
I’d like to help you, but I need more information. There are lots of reasons why you may be getting low fluorescence. It could be that you aren’t collecting enough vesicles, and you need to use more apoplastic wash in you experiments. It could be that you are losing your pellet during the centrifugation steps by using a different kind of tube or pipetting technique. Maybe your fluorometer has a different level of sensitivity or your DIOC has gone bad.
If you share your protocol with me. Maybe I can see what could be the problem.
Best of luck,
Brian
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