Dear Mr. BurbyI currently try to perform the spacer integration...

TZ
Tobias Zumbusch
Jul 20, 2018
protocol Protocol: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
Dear Mr. Burby
I currently try to perform the spacer integration in pPB105 for my bachelor project. It never worked so i have the question wether you had any Problems with this step (Golden Gate Step)? I always get unexplicable results after plasmid digestion of possible clones. The
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