Hello，I just followed ur protocal,but I met some problems,my...

Protocol: Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells

Hello，I just followed ur protocal,but I met some problems,my crypt cells vacuolated in the first day,but they can't bud,then they apoptosis in the next few days.I don't know if there is a problem with the use of my medium,How do you save them and add cell growth factors?Please kindly help Read more Read less

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