Does the media proteins/peptides interfere with preparation...

MZ
Ming Zhao
Jul 3, 2018
protocol Protocol: Isolation of the Secretome from Bacillus subtilis
Does the media proteins/peptides interfere with preparation of secretome ?
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ziba babazadeh fardi Answered Mar 8, 2019

university of maragheh

yes . it may interfere so you can use a blank (medium without inoculation of bacteria)and then do assay on both media. then calculate the secreted protein by bacteria
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