Improve Research Reproducibility A Bio-protocol resource

Dear Mrs Sharma,I would like to investigate stomata of conifers....

María del Carmen Dacasa Rüdinger Jun 29, 2018

Protocol: Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface

Dear Mrs Sharma,

I would like to investigate stomata of conifers. I found your protocol and think it could be appropriate to prepare the neddles. Maybe you have some experience with this or you know from other researchers that succeeded in the application of your protocol to con Read more more

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María del Carmen Dacasa Rüdinger Answered Jul 5, 2018

Division of Forest Genetics and Forest Tree Breeding, Wood and Forestry Competence Centre, Public Enterprise Sachsenforst, Germany, Germany,

Dear Nidhi,

thank you very much for your answer! I let you know, if we try the protocol and how it works with the visualization of the Stomata.

Kind regards,
María.

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Nidhi Sharma Author Answered Jul 5, 2018

HHMI/Stanford University

Hi Maria,
Thanks for contacting me about the protocol. Unfortunately, I have not tried clearing pine needles. The morphology of pine needles might make it harder to visualize stomata unlike Arabidopsis leaves. I would suggest trying out the protocol because clearing of the tissue would work in my opinion. I only foresee problems in visualizing stomata. Please let me know how it works out!
Best,
Nidhi.

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