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How can i get ficoll paque medium. if what i have is ficoll...

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Joseph Salifu David Jun 21, 2018
protocol Protocol: Isolation of Human PBMCs
How can i get ficoll paque medium. if what i have is ficoll pm 400 in grams how do i prepare it for use?
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Santosh K Panda Author Answered Jun 21, 2018

Infectious Disease Biology, Institute of Life Sciences, India

You can dissolve the Ficoll paque in isotonic sucrose. Follow the protocol provided by the supplier. As of my knowledge 1-1.2 gm/ml should be good for PBMC isolation.
here is a link for the product and procedure.
Thanks
https://www.sigmaaldrich.com/technical-documents/protocols/biology/ficoll-400.html
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