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Thank for your protocol. I have some quenstion： 1. the aims...
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qiulan lv Jun 9, 2018
Thank for your protocol.
I have some quenstion：
1. the aims of remove the water overnight to starve the mice ?
2. there are effects on weights between control and treated mice if i remove the water , so is it necessary to remove the water?
Thanks for your answers.
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Jalaj Gupta Author Answered Jun 16, 2018
Stem Cell Research Center (SCRC), Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow
Hi,
1) In our experience when we removed water (overnight or 4 to 6h before FITC-dextran), we see less variability in FITC-dextran concentration in bood in WT mice; probably because of even absorption of FD4.
2) we never did FD4 measurements in treated mice. We used untreated WT or KO mice and weight loss due to water removal for overnight or 4-6h was very minimal. I would suggest to remove water (you can reduce the time to 2-3h) only if treated mice are healthy.
I hope this will help you,
Good luck,
Jalaj
1) In our experience when we removed water (overnight or 4 to 6h before FITC-dextran), we see less variability in FITC-dextran concentration in bood in WT mice; probably because of even absorption of FD4.
2) we never did FD4 measurements in treated mice. We used untreated WT or KO mice and weight loss due to water removal for overnight or 4-6h was very minimal. I would suggest to remove water (you can reduce the time to 2-3h) only if treated mice are healthy.
I hope this will help you,
Good luck,
Jalaj
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