Hello, could you tell me how to Keep PBMC's activity in vitro...

Protocol: PBMC-MSC Co-cultures for Induction of Treg Generation

Hello, could you tell me how to Keep PBMC's activity in vitro for almost 7 days without any stimulation? I mentioned that Melidf, S.M. just culture PBMC alone in RPMI medium (containing P/S, L-glutamine and 10% FCS) after co-cultured with MSC in the reference at the end of this protocol. Read more Read less

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