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also can i Ask if i can do RNase R treatment during the protocol...

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walaa moustafa nassif May 27, 2018
protocol Protocol: Detection and Analysis of Circular RNAs by RT-PCR
also can i Ask if i can do RNase R treatment during the protocol of mirneasy extraction kit after separation of aqueos phase from qiazole and chloroform ??to save the extraction kits used again after rnase R treatment ?
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Amaresh Chandra Panda Author Answered May 28, 2018

Institute of Life Sciences Bhubaneswar

Hello,
We have not tried using RNase R in the aqueous phase from Qiazol preparation. The buffer condition in the aqueous phase may not be suitable for RNase R digestion. If you want to save a column, after RNase R treatment you may use phenol chloroform extraction method and precipitate the RNA using co-precipitant like Glycogen or Glycoblue (ThermoFisher SCientific).
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