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xueqin xu
May 18, 2018
protocol Protocol: Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
尊敬的老师:您好! 个人因为最近需要做N6-甲基腺苷RNA修饰水平的斑点印迹分析看过该protocol,觉得非常实用。然后综合多篇文献里提及的此实验,比如Yoon, K.J., et al., Temporal Control of Mammalian Cortical Neurogenesis by m(6)A Methylation. Cell, 2017. 171(4): p. 877-889.e17.发现该protocol缺少证实上样量一致的亚甲蓝染色实验。可是在实践操作过程中,每次ECL曝光完毕染色亚甲蓝并洗脱的过程中无法看到那 Read more Read less more less
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