Improve Research Reproducibility A Bio-protocol resource

Thanks, Tonya, for your response. Just to add, the concentration...

Moriya Tsuji May 17, 2018

Protocol: Generation of Human iNKT Cell Lines

Thanks, Tonya, for your response. Just to add, the concentration of CD14- cells is approx. 2.5 x 10^6/mL, meaning that 5 x 10^7/20 mL in T-75 flask.

Recombinant human IL-2 conversion is usually 2 x 10^4 IU/ug.

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Burcu İlçe Answered May 18, 2018

Ankara University

Thank you very much Moriya and Tonya.
Isn't the source of the cytokines that are used important? I thought it should be from human like HEK cells. As I saw you used E.coli origined IL-2, GMCSF etc. It isn't important at that point, right?

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