Usually we don't count the cells before stimulation, as they...

Holden T. Maecker May 6, 2018

Protocol: Intracellular Cytokine Staining on PBMCs Using CyTOF^TM Mass Cytometry

Usually we don't count the cells before stimulation, as they are already plated, and some may have adhered to the wells. However, we recommend to count and adjust the concentration just prior to running on the CyTOF instrument, so as to ensure an acquisition rate of 200-300 events per sec Read more Read less

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