hi,we have recently obtain Hpa.Noco from other lab and trying...

溟 何
Apr 23, 2018
protocol Protocol: Hyaloperonospora arabidopsidis (Downy Mildew) Infection Assay in Arabidopsis
hi,we have recently obtain Hpa.Noco from other lab and trying to use pad4-1 mutants for Hpa propagation. On the 4th day post inoculation we found necrosis-like spots on some of the leaves, and actually parts of some leaves are missing, which does not seem to be eaten by worms because these img Read more Read less more less
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溟 何 Answered Apr 23, 2018

华南植物园

both Hpa and pad4 mutants were obtained from the other lab. the infected plants were in a 50mL tube so we are not sure about the contamination problem, and pad4 are Col-0 mutant as so we were told.and yes we use sterile water without surfactants.
thx for your reply. I would take it as that our experiment somehow went wrong and try to find out the reason.
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Shuta Asai Author Answered Apr 23, 2018

Center for Sustainable Resource Science, RIKEN

One possibility is that your conidiospore solution contains contamination of other pathogens (e.g. necrotrophs).
Avoid any soil contaminations when you harvest aerial parts of the infected plants because it may cause propagation of soil inhabiting pathogens.
Are you sure the plant is a Col-0 pad4 mutant?, you use water without any surfactants?
Hope that your experiment goes well.
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