溟 何 Answered Apr 23, 2018
华南植物园
both Hpa and pad4 mutants were obtained from the other lab. the infected plants were in a 50mL tube so we are not sure about the contamination problem, and pad4 are Col-0 mutant as so we were told.and yes we use sterile water without surfactants.
thx for your reply. I would take it as that our experiment somehow went wrong and try to find out the reason.
Shuta Asai Author Answered Apr 23, 2018
Center for Sustainable Resource Science, RIKEN
One possibility is that your conidiospore solution contains contamination of other pathogens (e.g. necrotrophs).
Avoid any soil contaminations when you harvest aerial parts of the infected plants because it may cause propagation of soil inhabiting pathogens.
Are you sure the plant is a Col-0 pad4 mutant?, you use water without any surfactants?
Hope that your experiment goes well.