If experiment contains ~70 plants thus procedure from sampling...

溟 何
Apr 16, 2018
protocol Protocol: Surface Inoculation and Quantification of Pseudomonas syringae Population in the Arabidopsis Leaf Apoplast
If experiment contains ~70 plants thus procedure from sampling to serial dilution for all plants takes more than 4 hours, would it lead to more variable results due to Pseudomonas syringae proliferation in 10 mM MgCl2? Is it recommended to devide plants into several groups and repeat the w Read more Read less more less
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溟 何 Answered Apr 16, 2018

华南植物园

Thanks a lot.
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Cristián Jacob Author Answered Apr 16, 2018

Department of Plant Sciences, University of California, USA

Hello,

Yes, it is recommended to split the number of sample. We do not do more than 6-7 genotypes. You could start the different groups of plants in different days and do the experiments with plants of a same age or start all the plants at the same time and use plants that differ only in a couple of days of age. If you have a control (i.e.: wild type) add it to every experimental batch.
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