Am trial this protocol,but i used the complete media in step...

protocol Protocol: Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
Am trial this protocol,but i used the complete media in step of isolation (DMEM+penicillin-streptomycin+L glutamine +FBS) but not FBS MSC qualified ,is this true .I don't now why this protocol failed with me . I need to know what is false in my work.Please help me thanks very much.
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