I wonder how to label a peptide and a small molecule in order...

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ZHONGLIN CHAI
Apr 10, 2018
protocol Protocol: Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
I wonder how to label a peptide and a small molecule in order to perform BRET? Would it be an issue that the relative large tag molecule such as RLuc or YFP might affect the behavior of a short peptide or a small molecule?
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ZHONGLIN CHAI Answered Apr 11, 2018

ZHONGLIN CHAI

Great. Thank you for the useful information.
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Tinghai Xu Author Answered Apr 11, 2018

Saint Louis Universtiy

You can tag the peptide or a small molecule with Alexa 488 fluorophore, similar wavelength as YFP and can do BRET with Rlu tagged proteins.
It is a challenge to label the peptide with Rlu or YFP.
I hope this can help you.
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