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I was wondering what concentration of Digitonin used in the...

HZ
Hua Zhu Apr 10, 2018
protocol Protocol: Isolating Brain Mitochondria by Differential Centrifugation
I was wondering what concentration of Digitonin used in the isolation and how many minutes of its incubation on Ice?

Thank you very much in advance !

Hua

huazhu@pennmedicine.upenn.edu
UPENN
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Javier Traba Author Answered Apr 20, 2018

Universidad Autónoma de Madrid

Dear Hua Zhu,

In our experiments (mainly respiratory assays or calcium retention capacity assays), we added 100 micromolar digitonin to the assay buffer. Digitonin was present during the whole experiment.

Hope that helps. Best,

Javier
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