If the reagents have been prepared fresh each time and only...

Protocol: A Modified Chromogenic Assay for Determination of the Ratio of Free Intracellular NAD⁺/NADH in Streptococcus mutans

If the reagents have been prepared fresh each time and only prior to the color development section of the assay, then it should work as described. The reaction is extremely light sensitive; however, the exposure of the reagents to light would allow for a quicker, darker color development, Read more Read less

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