what is difference between isolation MSC media & MSC growth...

protocol Protocol: Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
what is difference between isolation MSC media & MSC growth media ,when iam work ,I use same media(complete media prepared as DMEM+penicillin strepto1%+FBS10%+1%glutamine) , I cant know whats difference.thanks
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Malancha TA Author Answered Apr 11, 2018

Department of Biological Sciences, IISER, India, India,

The isolation medium contains antibiotic-antimycotic (as fresh human tissue is being plated in this step). The MSC growth medium, which is used to culture the MSCs which have come out of WJ explants, contains pen-step. This is the only difference between these two media compositions.
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