Hi! I tried with the Calreticulin exposure experiment with MC38...

MD
Mai Duong
Apr 4, 2018
protocol Protocol: In vitro Assays for the Detection of Calreticulin Exposure, ATP and HMGB1 Release upon Cell Death
Hi! I tried with the Calreticulin exposure experiment with MC38 cells but even in the none-treatment group, the signal of CRT detected by FACS still very high, then it was almost similar to the treated cells. Could you please explain this issue to me? Thank you very much.
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ren luo Answered Nov 7, 2018

shantou university

Did you exclude the debris before next gating?
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