Hi, Fang I stored the cytoplasm Fraction after step 4 and did...

protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Hi, Fang I stored the cytoplasm Fraction after step 4 and did steps 5 to 8 as your protocol says to. to obtain the nuclear fraction, but my western blot showed a little of contamination from nucleus to cytoplasm and vice versa. I used anti UGPase to evaluation of cytoplasm and anti Histon Read more Read less more less
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Fang Xu Author Answered Feb 22, 2018

Shandong University

Hi Gabriel, You can wash more times with NRBT to avoid the contamination from cytosol. To minimize contamination from nuclear fraction, try to do the whole process quickly and gently to avoid the damage of the nuclear envelope.
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