Hi Fang, I am curious about that why the Triton was removed...

HC
HUAN CHEN
Feb 21, 2018
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Hi Fang, I am curious about that why the Triton was removed in Step 7 using NRB? Thanks,
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HUAN CHEN Answered Apr 11, 2018

Shanghai Jiao Tong University

Thank you!
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Fang Xu Author Answered Feb 22, 2018

Shandong University

Hi Huan, If you want to stock the nucleus, it's better to remove the triton to avoid any potential damage to the nuclear envelope.
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