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Hi Fang, I want to purify the membrane protein and nuclear...

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Anjil Kumar Srivastava Feb 16, 2018
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Hi Fang, I want to purify the membrane protein and nuclear protein to see the difference between the interaction of two proteins. What changes I can made to get the membrane proteins. Thanks Anjil
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Fang Xu Author Answered Feb 16, 2018

Shandong University

Hi Anjil, You just save the cytosolic potion and do another centrifugation at16000g to get rid of other pellet. The supernatant is then subjected to ultra-centrifugation(100000g for 1hr), by which the membrane will be pellet down. All the best! Fang.
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