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Do you dilute your venous blood prior to layering on top of...

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Deyin D Hsing Feb 1, 2018
protocol Protocol: Isolation of Human PBMCs
Do you dilute your venous blood prior to layering on top of the ficoll? Thank you!
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TANMOY NANDI Answered Aug 19, 2021

NIMHANS

You can use RPMI instead of PBS for Dilution
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Deyin D Hsing Answered Feb 14, 2018

Weill Cornell Medical College

Thank you!
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Santosh K Panda Author Answered Feb 14, 2018

Infectious Disease Biology, Institute of Life Sciences, India

Yes we diluted the blood with PBS before layering. For better harvest dilute 1:1 ratio for small amount of blood like 5-10 ml. For large volume like 50 ml you could go for 2:1 blood and PBS ratio.
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