Please can you tell me in which proportion I will mix the extraction...

AR
Anindita Roy
Jan 26, 2018
protocol Protocol: In vitro Colorimetric Method to Measure Plant Glutamate Dehydrogenase Enzyme Activity
Please can you tell me in which proportion I will mix the extraction and reaction buffer agents in the method of In vitro Colorimetric Method to Measure Plant Glutamate Dehydrogenase Enzyme Activity ? Please reply me ASAP its urgent.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Daniel Marino Author Answered Jan 26, 2018

Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Spain

As described in Procedure, point D. It is usual to determine the activity out of 20 microliters of extract adding 280 microliters of reaction buffer. However, in function of the species, the tissue and the developmental stage GDH activity is different. Thus, if the activity is very high you may need to dilute the sample to obtain a linear kinetic. On the contrary, if it is too low you may need to concentrate the sample.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer