Daniel Marino Author Answered Jan 26, 2018
Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Spain
As described in Procedure, point D. It is usual to determine the activity out of 20 microliters of extract adding 280 microliters of reaction buffer. However, in function of the species, the tissue and the developmental stage GDH activity is different. Thus, if the activity is very high you may need to dilute the sample to obtain a linear kinetic. On the contrary, if it is too low you may need to concentrate the sample.