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Dear Author, I would like to know whether the DNase treatment...

Runlai Hang
Runlai Hang Dec 19, 2017
protocol Protocol: Polysome Analysis
Dear Author, I would like to know whether the DNase treatment is needed for the RNA before further RT reaction?
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Dipak Kumar Poria Author Answered Dec 20, 2017

National Cancer Institute

Hello Runlai, DNase digestion is not necessary for the RNA before RT reaction as cytoplasmic fraction is used for the polysome analysis in this protocol. Cell lysate preparation step in this protocol removes nucleus from the cytoplasmic fraction. Best, Dipak
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