Hello, For my thesis research I would like to stain young tomato...

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Vicky Everaerts
Oct 30, 2017
protocol Protocol: Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
Hello, For my thesis research I would like to stain young tomato leaf petioles. I can only find DAB staining protocols on leaves. I don't know if it might be possible to stain larger plant organs. Do you have an idea if it has ever been done before and if it is even possible? I would pro Read more Read less more less
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Arsalan Daudi Author Answered Nov 19, 2017

University of California

Hi Vicky, Thanks for reaching out- I think it should be doable, I didn't try this method on tomato petioles, but I know colleagues over the years who have tried a modified version of this protocol on non leaf tissue. It is worth trying to see if at least you get a DAB stain. Try de-staining a little longer, and yes a dilution series with DAB could help too. I'd be interested to hear about how it turns out for you.
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