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1.what degree can polarization inducing influence the intracellular...

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xiaoyun lin Oct 29, 2017
protocol Protocol: Intracellular Cytokine Staining (ICS) on Human Lymphocytes or Peripheral Blood Mononuclear Cells (PBMCs)
1.what degree can polarization inducing influence the intracellular cytokine expression before PMA/ionomycin stimulating. 2.Is there any relation between the activator concentration and different cytokine expression. 3.Could this protocol apply to a mice PBMCs smaple.
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Holden T. Maecker Author Answered Oct 29, 2017

Immunity, Transplantation & Infection, School of Medicine, Stanford University, Stanford

Short-term stimulation with PMA+ionomycin should not affect polarization of cells to Th1, Th2, etc. it should just reveal the existing potential of the cells. Similarly, I don't think stimulus concentration should differentially affect cytokine readouts, though we haven't rigorously tested this. The protocol should be adaptable to mouse cells, with appropriate mouse-specific antibodies.
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