Gel does not harden. Why? Gel mixture =culture medium (60...

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kyungHwa Cho
Sep 23, 2017
protocol Protocol: 3D Gel Invasion Assay of Gastric Cancer Cells with Fibroblasts
Gel does not harden. Why? Gel mixture =culture medium (60 μl)+reconstitution buffer (60 μl) +type-I collagen (480 μl) + matrigel (200 μl). In addition, I did not understand this part. What are the ‘at first prepare 600 μl of type-I collagen by mixing 10x conc' Please ask your answer.
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Masamitsu Tanaka Author Answered Sep 23, 2017

Department of Molecular Medicine and Biochemistry, Akita University Graduate School of Medicine, Japan

Please put in a tube as following order on ice. Culture medium (10x conc, 60 μl) Reconstitution buffer (60 μl) Type-I collagen (480 μl) . Mix well. Then, add 200 μl of matrigel matrix and mix thoroughly.
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