Gel does not harden. Why?
Gel mixture
=culture medium (60 μl)+reconstitution buffer (60 μl) +type-I collagen (480 μl) + matrigel (200 μl).
In addition, I did not understand this part. What are the ‘at first prepare 600 μl of type-I collagen by mixing 10x conc'
Please ask your answer.
Department of Molecular Medicine and Biochemistry, Akita University Graduate School of Medicine, Japan
Please put in a tube as following order on ice.
Culture medium (10x conc, 60 μl)
Reconstitution buffer (60 μl)
Type-I collagen (480 μl) .
Mix well.
Then, add 200 μl of matrigel matrix and mix thoroughly.