Dear teacher:
Thank you for the protocol you have provided.When I have done the In Vivo Matrigel Plug Angiogenesis Assay following your protocol. After remove the matrix glue form the mouse, fixed with formalin overnight, and then embed in paraffin.And this step is what I do not undersRead moreRead less
You are right, after formalin fixation, the gel plug has to go through the serial ETOH dehydration and xylene treatment, just like preparation of other normal/tumor tissues.