Dear teacher: Thank you for the protocol you have provided.When...

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Sep 19, 2017
protocol Protocol: In vivo Matrigel Plug Angiogenesis Assay
Dear teacher: Thank you for the protocol you have provided.When I have done the In Vivo Matrigel Plug Angiogenesis Assay following your protocol. After remove the matrix glue form the mouse, fixed with formalin overnight, and then embed in paraffin.And this step is what I do not unders Read more Read less more less
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HongLok Lung Author Answered Sep 28, 2017

University of Hong Kong

You are right, after formalin fixation, the gel plug has to go through the serial ETOH dehydration and xylene treatment, just like preparation of other normal/tumor tissues.
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