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Am I missing where you are using the Wnt3a conditioned media?...

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Kathryn Kilpatrick Sep 8, 2017
protocol Protocol: Wnt Reporter Activity Assay
Am I missing where you are using the Wnt3a conditioned media? I added it to my 293t-OT cells and they are not fluorescing at all.
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afshan iqbal Answered Feb 11, 2019

Institute of Biochemistry & Biotechnology

Hi,
I got nice results from the experiment. I put my Wnt CM on transfected HEK 293T cells. Cells were transfected with TOP and FOP plasmids.
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