Can I use GFP-antibody to improve green florescence and then...

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Wu Jia Sian
Sep 7, 2017
protocol Protocol: Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
Can I use GFP-antibody to improve green florescence and then use PI stain to detect cell cycle? Any protocol can I follow?
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Hui Zhu Author Answered Sep 7, 2017

Stanford University

I have used GFP antibody in Immunofluorescence staining. For FACS, I have never tried. If GFP+ % is fairly high, you don't need to add antibody. If GFP+ % is very low, adding antibody may not help.
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