请教林教授:...

婷 王
Sep 4, 2017
protocol Protocol: Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
请教林教授: 我现在进行T淋巴细胞活化相关实验。包括检测T淋巴细胞内BATF的表达量,在活化的T淋巴细胞上其表达增多。还需要检测TH1,TH2,TH17包内淋巴因子的含量,请教您为刺激T淋巴细胞活化我需要应用PMA/ionomycin还是CD3/CD28 ? 国外文献中刺激T细胞活化用CD3/CD28,只有单独检测胞内细胞因子时采用PMA/ionomycin。是这样么?
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Holden T. Maecker Author Answered Sep 4, 2017

Immunity, Transplantation & Infection, School of Medicine, Stanford University, Stanford

Thanks for your interest in the protocol. You can use either CD3/CD28 or PMA/ionomycin to stimulate Th1, Th2, and Th17 cytokine secretion from T cells. The difference is that PMA/ionomycin will stimulate many more T cells, as well as NK cells, monocytes, etc. With CD3/CD28, a smaller fraction of T cells respond, and you may not see the more rare cytokines like IL-4.
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