HelloPlease could you advise me as to how to prepare slides...

SF
Sarah Fernandes
Aug 15, 2017
protocol Protocol: Infiltration of Nicotiana benthamiana Protocol for Transient Expression via Agrobacterium
Hello

Please could you advise me as to how to prepare slides for N benthamiana for fluorescent microscopy. Is it necessary to use a microtome or can one peel the epidermis away? My sample preparation will be made a bit more challenging as I will be dehydrating the plants for abo
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kaushik ghose Answered Sep 16, 2019

kaushik ghose

I have used this protocol for BiFC and it worked perfectly. I agreed with Marek. Just a piece of leaf suspended in water is good enough. Make sure you water the plants well before infiltration. I normally kept the plants in dark between 3-4 days before monitoring the fluorescence.
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Marek Szecowka Answered Sep 14, 2017

University of Cambridge

It depends on what you are trying to see. For observation of fluorescent proteins usually you can use small piece of a leaf and suspend it in watter. You don't have to remove an epidermis.
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