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Why do crystals form? Do they form in the staining solution...

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Nathan Tang Jul 11, 2017
protocol Protocol: Senescence Associated β-galactosidase Staining
Why do crystals form? Do they form in the staining solution or when the staining solution was applied? Also, why do you have to wait 5 days after you have induced senescence in order to stain? Does that mean that the cells go through 5 divisions and then become senescent? Because if the c Read more Read less more less
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Vikas Prakash Ranvir Answered May 3, 2021

Advanced Centre for Treatment, Research and Education in Cancer

Crystals develop if the staining solution evaporates from the wells.
To prevent this, seal your plates with ParaFilm.
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