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why only the choromosomal DNA only precipitates but not the...

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suprita panigrahi Jun 30, 2017
protocol Protocol: Plasmid DNA Extraction from E. coli Using Alkaline Lysis Method
why only the choromosomal DNA only precipitates but not the plasmid DNA?
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Suprim Tha Answered Oct 15, 2017

Tribhuwan University

That's because during the renaturation step ie, during addition of solution III (Pot. acetate solution), smaller plasmid DNA renatures fast but long chromosomal DNA cannot. Also these single stranded DNAs along with other residual protein parts due to hydrophobic interactions coagulate to form a white precipate which is pelleted out after this step.
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