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Why did you choose to use 0.22 um filters? OMVs can range in...

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Tiffany Lowe-Power Jun 23, 2017
protocol Protocol: Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris
Why did you choose to use 0.22 um filters? OMVs can range in size from 30-300 nm, so these filters might exclude the larger vesicles.
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Ofir Bahar Author Answered Jun 25, 2017

Agricultural Research Organization, Volcani Center

Your point is absolutely true.We had to decide whether we want to be more stringent with the filtering by using the 0.22uM, on the cost of loosing some large OMV, or use 0.45uM and then risk that some contaminants will go through the filter. Since we had some contamination issues in the past while using the 0.45uM we decided be on the safe side with the filtering.
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