We forgot to rinse our samples that had been in PFA with PBS...

protocol Protocol: Mouse Cochlear Whole Mount Immunofluorescence
We forgot to rinse our samples that had been in PFA with PBS before adding the EDTA to decalcify. Do you think this will make a big difference to our samples? And if so, do you know of anything we can do to fix the problem?
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Omar Akil Author Answered Jun 21, 2017

University of California, San Francisco

If you forgot to rinse your samples from PFA there will be no negative effect to your samples. For your information some protocols use 1% PFA with EDTA when decalcifying.
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