We forgot to rinse our samples that had been in PFA with PBS before adding the EDTA to decalcify. Do you think this will make a big difference to our samples? And if so, do you know of anything we can do to fix the problem?
If you forgot to rinse your samples from PFA there will be no negative effect to your samples. For your information some protocols use 1% PFA with EDTA when decalcifying.