Hi, I am doing trypan blue staining in Nicotiana benthamiana...

Jinping Zhao
Jinping Zhao
Jun 19, 2017
protocol Protocol: Plant Tissue Trypan Blue Staining During Phytopathogen Infection
Hi, I am doing trypan blue staining in Nicotiana benthamiana according to the protocol. In the Recipe of staining buffer it says that the final concentration of trypan blue is 10 mg/ml (1%), however 40 mg of trypan blue resolved in the liquid mixture makes a concentration of 1 mg/ml (0.1%) Read more Read less more less
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xiao Zhang Answered Aug 30, 2017

Chinese Academy of Agricultural Sciences(CAAS)

您好,我叫张晓,请问你也在用台酚蓝染色的方法看烟草的cell death,请问可否分享一下这个方法的经验?
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Marta Berrocal-Lobo Author Answered Aug 23, 2017

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Parque Científico y Tecnológico, UPM, Campus de Montegancedo, Spain

Dear user,
You can try with 0,04g in 40ml of mix, this is the minimum for Arabidopsis leave staining, however with Nicotiana you might need more, the leaves are thicker.
Let me know your results. I will review the text to fix some error.
We observed with Nicotiana sometimes some whitering on leaves during ethanol step.
Good luck
Marta
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