Hi,I have been trying to determine Arabidopsis leaf tissue nitrite...

ZJ
Zhe Ji
Jun 14, 2017
protocol Protocol: Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
Hi,
I have been trying to determine Arabidopsis leaf tissue nitrite (NO2-) with your protocol. I used 100 mg of wild type leaf tissue and followed each step but have got no color development at the end of the experiment. The standard curve worked fine so it's not likely due to the col
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John J Smith Answered Oct 27, 2018

Harvard Business School

Hello Zhe Ji I need access to ur paper lol XDAB
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Takushi Hachiya Author Answered Jun 15, 2017

Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences,, Nagoya University, Japan

Dear Dr. Luke,

Tissue nitrite concentration is often very low under normal growth condition in wild-type plants, because toxic nitrite is rapidly converted into ammonium by NiR. A significant detection may be made in the plants grown under hypoxia/anoxia or other nitrite-accumulating/containing conditions or in the mutants showing lower nitrite reduction.
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