Hi,Right now I am trying to doUV mutagenesis for powdery mildew...

YW
Ying Wu
May 29, 2017
protocol Protocol: Measuring UV-induced Mutagenesis at the CAN1 Locus in Saccharomyces cerevisiae
Hi,

Right now I am trying to doUV mutagenesis for powdery mildew (a biotroph fungi). However, I am not sure what machine and dose I should use. Could you tell me the UV machine you used? I have a UV-crosslinker. The power is 10,000uJ/cm2. Do you think if I can use it for mutagen
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Ildiko Unk Author Answered Aug 24, 2017

The Institute of Genetics, Biological Research Center of The Hungarian Academy of Sciences, Hungary

Hi,
The machine was made here at the institute according to my request.
I have never worked with powdery mildew, so you have to search the literature, or try different doses.
In my experience, UV cross linkers do not work well for irradiating fungi on plate, because they can only irradiate with one single dose, but you usually have to apply a broad range of doses to perform an experiment, e.g. a killing curve.
My advice is to look for a lab in/around your institute that already has a machine you need.
Best,
Ildiko
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