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Dear Fang,I want to extract nuclear from Arabidopsis using the...

JL
Jun Li Apr 27, 2017
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Dear Fang,
I want to extract nuclear from Arabidopsis using the protocol which is highly similar with yours. I want to make Lysis buffer without DTT and PMSF, and what temperature can store the Lysis buffer (without DTT and PMSF) ? How can I sterilize the Lysis buffer without DTT and Read more Read less more less
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Fang Xu Author Answered Aug 23, 2017

Shandong University

Hi Jun, sorry for missing your questions,you can autoclave your lysis buffer without adding DTT, PMSF and PI. Also I would think it's not a big problem if you directly use the EDTA ph=8. Hope this is still helpful for you. Thanks.
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