Hi! I'm very grateful with you for share this protocol, i've...

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catalina rubio
Apr 19, 2017
protocol Protocol: Senescence Associated β-galactosidase Staining
Hi! I'm very grateful with you for share this protocol, i've been testing it with different Line cells like T98g and Stem cells.

I'm agree with you this assay it is best to avoid over-confluency of the cells, and will have a better staining , and at the beginning I had so
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yan huang Answered Dec 5, 2019

Sun Yat-sen university

I tried to use ethanol to remove the crystals and it works, 3-5 hours enough, hope to help you.
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Michael Eccles Author Answered Apr 19, 2017

Department of Pathology, University of Otago, New Zealand

Thanks very much for this.
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