Like all other quantitative analysis with fluorescence, it needs...

protocol Protocol: Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag
Like all other quantitative analysis with fluorescence, it needs to carefully decide the proper dilution rates of the samples in order to increase the accuracy in the linear intensity-concentration range.
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Marielle Cavrois Author Answered Mar 9, 2017

Gladstone Institutes and University of California, San Francisco

Yes, the FlaQ assay is designed to quantify p24Gag in solutions ranging from 10 ng/ml to 78.12 pg/ml.
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