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Hi,Thanks for posting this protocol. I find this very helpful...
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Fatima Zia Mar 3, 2017
Hi,
Thanks for posting this protocol. I find this very helpful for my studies. However, I have following few questions:
1) I will be performing this assay with leaves of Arabidopsis seedlings (12-day old). In that case, which multiwell plate do you think would be best Read more
Thanks for posting this protocol. I find this very helpful for my studies. However, I have following few questions:
1) I will be performing this assay with leaves of Arabidopsis seedlings (12-day old). In that case, which multiwell plate do you think would be best Read more
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Arsalan Daudi Author Answered Apr 24, 2017
University of California
Hi Fatima,
I recommend 12-well for Arabidipsis - any smaller will likely not be appropriate
I use pipettes for step 22 and replacing the bleach and de-stain solutions - it is easier and less likelihood of tissue damage
For storage, diluted destain solution is OK at 4C
Yes additional stabilization in water for 24 h is a good idea if you cut leaves. This is standard for leaf disc assays for example.
Good luck and let us know how it goes!
Please share progress of your experiemnt using the feedback option
I recommend 12-well for Arabidipsis - any smaller will likely not be appropriate
I use pipettes for step 22 and replacing the bleach and de-stain solutions - it is easier and less likelihood of tissue damage
For storage, diluted destain solution is OK at 4C
Yes additional stabilization in water for 24 h is a good idea if you cut leaves. This is standard for leaf disc assays for example.
Good luck and let us know how it goes!
Please share progress of your experiemnt using the feedback option
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