Arsalan Daudi Author Answered Apr 24, 2017
University of California
Hi Fatima,
I recommend 12-well for Arabidipsis - any smaller will likely not be appropriate
I use pipettes for step 22 and replacing the bleach and de-stain solutions - it is easier and less likelihood of tissue damage
For storage, diluted destain solution is OK at 4C
Yes additional stabilization in water for 24 h is a good idea if you cut leaves. This is standard for leaf disc assays for example.
Good luck and let us know how it goes!
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