Hi,I want to know about plasmid DNA isolation.this is my saminar...

Gulshan Kumar
Gulshan Kumar
Feb 15, 2017
protocol Protocol: Plasmid DNA Extraction from E. coli Using Alkaline Lysis Method
Hi,
I want to know about plasmid DNA isolation.this is my saminar topic.So can u please help me for this.I don't know much about this.is there any other methods for isolation of plasmid DNA.and can we use any other bacteria for isolating plasmid DNA.why we use E.coli there.please tell
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Suprim Tha Answered Oct 20, 2017

Tribhuwan University

Any other organisms could be used. This particular E.coli strain is used because of its availabilty, non-pathogenicity and mainly due to its antibiotic susceptible nature, which would make the transformants selection process more efficient.
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