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can i replace phosphoric with orthophosphoric acid?and if i...
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mahmoud ali Feb 11, 2017
can i replace phosphoric with orthophosphoric acid?
and if i can could i use it without dilution direct from bottle
and if i can could i use it without dilution direct from bottle
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Fanglian He Author Answered Feb 14, 2017
Bio-protocol
Hi,
Phosphoric acid and orthophosphoric acid are often considered as the same thing (http://www.sigmaaldrich.com/catalog/product/aldrich/345245?lang=en®ion=US).
The one mentioned in the protocol was 85% (w/v) from vendor. I did not dilute it. Whatever the concentration of your H3PO4 stock is, its final concentration in the reagent should be 8.5% (w/v).
Hope this helps.
Fanglian
Phosphoric acid and orthophosphoric acid are often considered as the same thing (http://www.sigmaaldrich.com/catalog/product/aldrich/345245?lang=en®ion=US).
The one mentioned in the protocol was 85% (w/v) from vendor. I did not dilute it. Whatever the concentration of your H3PO4 stock is, its final concentration in the reagent should be 8.5% (w/v).
Hope this helps.
Fanglian
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