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In the dye reagent's recipe, I wonder how much is the total...
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Le Nghia Oct 19, 2016
In the dye reagent's recipe, I wonder how much is the total volume of reagent. Is it about 150 ml, include 50 mg Coomassie G250, 50 ml Methanol, and 100 ml 85% Phosphoric acid? Don't we need to add mQ to adjust final volume?
And I think Methanol is poisonous, so can I replace it by Et Read more
And I think Methanol is poisonous, so can I replace it by Et Read more
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Fanglian He Author Answered Feb 14, 2017
Bio-protocol
Hi Le,
Sorry to miss your questions. Yes, you were right that we should adjust the final vol.. As 50mg Coomassie G250 would not change the vol significantly, I did not write it in that way.
That is good point. I never got chance to try Ethanol. Please share your experience here if it works in your hand.
Thanks,
Fanglian
Sorry to miss your questions. Yes, you were right that we should adjust the final vol.. As 50mg Coomassie G250 would not change the vol significantly, I did not write it in that way.
That is good point. I never got chance to try Ethanol. Please share your experience here if it works in your hand.
Thanks,
Fanglian
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